Glass frogs comprise a family (Centrolenidae) of 158 species found in the forests of the neotropics: Central America and northern South America. They get their name from the transparency of their belly skin, which allows the internal organs to be easily seen. They are adapted to life in the trees, possessing pads on the tips of their digits that allow them to adhere to leaves. These structures appear to have evolved independently in several lineages of frogs, since molecular phylogenetic results show that the main families where these pads occur (Hylidae, Rhacophoridae, Centrolenidae) are not closely related.
One of the special features of glass frogs is their mode of reproduction. Clutches of eggs, as flat round sheets, each egg encased in jelly, are laid on the surfaces of leaves overhanging streams. Once the eggs have developed into larvae, they hatch and fall into the stream below, often from a considerable height. Glass frogs are divided into two main sub-families: the Centroleninae (121 species) which lay on the upper sides of leaves and then leave the eggs to develop on their own; and the Hyalinobatrachinae (35 species) which lay their eggs on the lower sides of leaves; in these species, the father cares for the eggs, sometimes up till the point of hatching.
We studied the glass frog Hyalinobatrachium orientale which has distinct populations in northern Venezuela and northeast Tobago: this distribution is a bit of a puzzle. Northeast Tobago is quite distant from Venezuela and between these two locations is the large island of Trinidad, which has abundant forests, but no glass frogs. Walking along the forest streams of Tobago at night, once the rainy season (June to December) has started, you soon hear the high-pitched peeping of male glass frogs, located on the huge leaves of Heliconia bihai that overhang the water. With the aid of a good torch, you can locate the calling frogs; often, near them, you can spot the little patches of eggs. If you are lucky, you may locate a mating pair. We observed the behaviour of a mating pair. It took them about four hours to complete their clutch of around 30 eggs, laid as a spiral pattern, starting at the centre, with the pair turning as they proceeded. Once egg-laying was complete, the female departed, but the male stayed close to the clutch. Often, when searching for egg clutches, we found the father sitting on top of his eggs. We also found that some fathers, presumably good quality males, were looking after more than one egg clutch at a time. These are at different stages of development, so clearly produced on different nights. It is not entirely clear what functions male egg attendance performs, particularly given that it is not constant. However, observers have seen males driving away egg predators such as wasps and ants; it is also likely that males keep the eggs hydrated by reducing evaporation, simply by sitting on them, or by emptying their bladders over the eggs (this is established in some cases of frog parental care). But this raises another mystery. If paternal care is helpful to incubation success in the Hyalinobatrachinae, why does it not occur in other glass frogs, especially when they lay their eggs on leaf upper surfaces, where you would guess that desiccation and predation would be higher risks.
In the Tobago glass frog, hatching occurs around nine days after laying, although the actual time is variable, allowing tadpoles to be earlier or later stages of development when entering the water. Such variability may be quite common in frog development and represents a trade-off. Early hatchers are less well developed and more vulnerable when they enter the water; but it may be better to risk this than to be predated while still in the nest. It therefore pays the developing larvae to monitor conditions: if the father has deserted his clutch, or hot sun and no rain are risking desiccation, better to hatch early and hope for a stream with few predators.
The streams where glass frog tadpoles are found are fast-flowing when it rains, and are heavily populated with predatory fish and crustaceans. They are also shaded, making plant productivity low. As a consequence, glass frog tadpoles spend much of their time hidden under rocks, reducing the risks of predation and of being swept away by currents (unlike the tadpoles of some species that inhabit fast streams, glass frog tadpoles lack the kind of suctorial mouthparts that can help cling on to rocks). The tadpoles have long muscular tails, indicating an ability to move rapidly, and are relatively unpigmented, associated with a concealed lifestyle. Their behaviour limits foraging opportunities, so growth is slow. Glass frog tadpoles can take a year to reach metamorphosis, very slow by tropical frog standards, where many species reach that stage in a few weeks. We have been able to locate metamorphosing glass frogs near the edges of streams. They climb up on to the upper surfaces of leaves close to the ground, and take about four days to complete the process, reducing their tail to a stump. To our surprise, we found that they do not remain in one place through this process, but occasionally move around, possibly to confuse potential predators.
The transparency of glass frogs has long puzzled biologists. Recently, a research team from Bristol, Canada and Ecuador has tested an explanation. Their evidence suggests that it is not so much transparency that matters, but translucence. When a glass frog is at rest with its limbs tucked in, the translucence of the limbs blurs the edges of the body, and makes detection by predators more difficult.
Further reading
Barnett et al. Imperfect transparency and camouflage in glass frogs. PNAS (2020).
Byrne et al. The behaviour of recently hatched Tobago glass frog tadpoles. Herpetological Bulletin 144, 1-4 (2018).
Byrne et al. Observations of metamorphosing tadpoles of the Tobago glass frog. Phyllomedusa (in press) (2020).
Delia et al. Glass frog embryos hatch early after parental desertion. Proceedings of the Royal Society B 281, 2013-2037 (2014).
Downie et al. The tadpole of the glass frog Hyalinobatrachium orientale from Tobago. Herpetological Bulletin 131, 19-21 (2015).
Nokhbatolfoghahai et al. Oviposition and development in the glass frog Hyalinobatrachium orientale. Phyllomedusa 14, 3-17 (2015).
Contributing authors : Roger Downie, Isabel Byrne, Chris Pollock.
